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Publication Details

Title :

Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua BasalisMesenchymal Stem/Multipotent Stromal Cells

Journal:

Stem Cells International

Impact Factor:

2.806

Authors:

T. Khatlani,1D. Algudiri,1 R. Alenzi,1A. M. Al Subayyil,1 F.Abomaray,2,3 E. Bahattab,4 A. S. AlAskar,1,5,6 B. Kalionis,7 M. F. El-Muzaini,8 and M. H. Abumaree1,9

Affiliations:

1Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi Arabia

2Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, 14186 Stockholm, Sweden

3Center for Hematology and Regenerative Medicine, Karolinska Institutet, 14186 Stockholm, Sweden

4National Center for Stem Cell Technology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia

5College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh 11481, Saudi Arabia

6Adult Hematology and Stem Cell Transplantation, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi Arabia

7Department of Obstetrics and Gynaecology and Department of Maternal-Fetal Medicine Pregnancy Research Centre, Royal Women’s Hospital, University of Melbourne, Parkville, VIC 3052, Australia

8Department of Obstetrics and Gynaecology, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh 11481, Saudi Arabia

9College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh 11481, Saudi Arabia

Year of Publication:

2018

DOI:

10.1155/2018/6480793

Abstract:

Stem cell-based therapies rely on stem cell ability to repair in an oxidative stress environment. Preconditioning of mesenchymal stem cells (MSCs) to a stress environment has beneficial effects on their ability to repair injured tissues. We previously reported that MSCs from the decidua basalis (DBMSCs) of human placenta have many important cellular functions that make them potentially useful for cell-based therapies. Here, we studied the effect of DBMSC preconditioning to a stress environment. DBMSCs were exposed to various concentrations of hydrogen peroxide (H2O2), and their functions were then assessed. DBMSC expression of immune molecules after preconditioning was also determined. DBMSC preconditioning with H2O2 enhanced their proliferation, colonogenicity, adhesion, and migration. In addition, DBMSCs regardless of H2O2 treatment displayed antiangiogenic activity. H2O2 preconditioning also increased DBMSC expression of genes that promote cellular functions and decreased the expression of genes, which have opposite effect on their functions. Preconditioning also reduced DBMSC expression of IL-1β, but had no effects on the expression of other immune molecules that promote proliferation, adhesion, and migration. These data show that DBMSCs resist a toxic environment, which adds to their potential as a candidate stem cell type for treating various diseases in hostile environments.