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Publication Details

Title :

The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks

Journal:

PLoS One.

Impact Factor:

2.766

Authors:

Alshareeda AT1, Rakha E2, Alghwainem A1, Alrfaei B1, Alsowayan B1, Albugami A1, Alsubayyil AM1, Abomraee M1, Mohd Zin NK3.

Affiliations:

1 Stem Cell and Regenerative Medicine Department, King Abdullah International Medical Research Centre, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Saudi Arabia.

2 University of Nottingham and Nottingham University Hospitals NHS Trust, City Hospital, Department of Cellular Pathology, UK, Nottingham, United Kingdom.

3 School of Advance Science and Engineering, Waseda University, Tokyo, Japan.

Year of Publication:

2018

DOI:

10.1371/journal.pone.0207593

Abstract:

Mesenchymal stem cells (MSCs) can influence the tumour microenvironment (TEM) and play a major role in tumourigenesis. Triple-negative [Ostrogen receptor (ER-), Progesterone receptor (PgR-), and HER2/neu receptor (HER2-)] breast cancer (TNBC) is an aggressive class of BC characterized by poor prognosis and lacks the benefit of routinely available targeted therapies. This study aims to investigate the effect of human placental chorionic villi derived MSCs (CVMSCs) on the behavior of TNBC in vitro. This was done by assaying different cancer hallmarks including proliferation, migration and angiogenesis. Cell proliferation rate of TNBC cell line (MDA-MB231) was monitored in real time using the xCELLigence system. Whereas, Boyden chamber migration assay was used to measure MDA-MB231 motility and invasiveness toward CVMSCs. Finally, a three-dimensional (3D) model using a co-culture system of CVMSCs with MDA-MB231 with or without the addition of human umbilical vein endothelial cells (HUVECs) was created to assess tumour angiogenesis in vitro. CVMSCs were able to significantly reduce the proliferative and migratory capacity of MDA-MB231 cells. Co-culturing of MDA-MB231 with CVMSCs, not only inhibited the tube formation ability of HUVECs but also reduced the expression of the BC characteristic cytokines; IL-10, IL-12, CXCL9 and CXCL10 of CVMSCs. These results support the hypothesis that CVMSCs can influence the behavior of TNBC cells and provides a basic for a potential therapeutic approach in a pre-clinical settings. The data from this study also highlight the complexity of the in vitro cancer angiogenesis model settings and regulations.